Plasmid_Backbone

Part:BBa_J61003:Design

Designed by: John Anderson   Group: Arkin Lab   (2006-07-31)


EN-Ptet-rbs-X-[GFP]-SNP "ORF Expresser"


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1097


Design Notes

N/A


Source

PCR KB001/ca1021R on pSB1A2-J01022 (250 bp, EcoRI/XbaI)

sub into pSB1A2-E0040(GFP;DNA-1-5H) (EcoRI/XbaI)

Product is pBca1021-E0040

ca1021R Reverse b0034 XbaI

AGCCATCTAGAATTTCTCCTCTTTCTCTAG

KB001 Forward EcoRI to construct Ptet part-making biobrick plasmid

ttctggaattcgcggccgcaactagagccaggcatc

References